A combined cytochemical and electron. microscopic study was carried out for the demonstration of acid phosphatase activities in trophozoites of E. histolytica. and E. gingivalis. E. histolytica(YS-27) strain was isolated from liver abscess of 72-year-old man in September 1969, and. E. gingivalis (YS-215)...strain was collected from gingival crevice of 41-year-old man´ in January 1972. The amoeba strains were maintained by subculture on diphasic medium, and used throughout the study.
The results are summarized as follows
1. In E. histolytica, the reaction products were distributed evenly over the entire surface of plasma membrane, whereas E. gingivalis showed no activity of acid phosphatase on the plasma membrane,. except in the portion of the uroid-like structure.
2. In the cytoplasm; various reaction precipitates were observed in vacuoles of both amoebae ; vacuole limiting membrane, vacuole membrane and its contents And lysosome-like structure. Strong enzyme active contents but membrane reaction negative vacuoles were conspicuous in E. gingivalis. Endoplasmic reticulum showed a moderate activity.
3. Granule-like acid phosphatase reaction. product was demonstrated in the .nucleoplasm of E. gingivalis, but it was negativin E. histolytica.
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